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Background
Bioscan is an international research program being conducted by the International Barcode of Life Consortium (iBOL) between 2019 and 2026 to speed species discovery, to probe species interactions, and to track species dynamics through use of DNA barcodes.
A core component of Bioscan is the Global Malaise Trap Program, which seeks to operate Malaise traps across a wide fraction of the world’s ecosystems to sample flying insects and other invertebrates and understand more of local species diversity and of global biodiversity patterns.
The Global Assessment Report on Biodiversity and Ecosystem Services released in 2019 by IPBES warns that the planet is undergoing a massive human-created extinction event and that our ability to respond to this crisis is hampered by our limited knowledge of biodiversity patterns and trends, particularly for less charismatic groups of organisms. There is widespread evidence that insect numbers have been collapsing in several regions of the world, but we have few baselines studies that can help us to measure these changes. Scientific monitoring of insect communities and populations is now more important than ever. Malaise trapping within Bioscan can help to fill our knowledge gaps.
These Araba Bioscan Project pages document the operation of a Malaise trap in Aranda, a suburb of Canberra Australia.
Location

The trap was originally positioned below an Ironbark (Eucalyptus sideroxylon A.Cunn. ex Woolls) in a suburban garden which also contains a large Red Box (Eucalyptus polynathemos Schauer).

During the first three weeks of the project, the proportion of Lepidoptera in the samples was surprisingly high and insects from other groups were collected only in low numbers, apparently a result of the specific positioning of the trap. A SLAM trap was erected around 10 m from the position of the Malaise trap in a slightly more open location and nearer to a ridge. The results from a five day test were promising with many more Coleoptera and Blattodea and a lower overall proportion of Lepidoptera.

As a result, a second Malaise trap was put up close to the position used for the SLAM trap. The two Malaise traps operated in parallel for a week for a more direct comparison, following which the original trap was dismantled. The samples from the first four weeks at the original position will be sequenced to validate my sample handling.

The location of the property is around 200 m from the Aranda Bushland Nature Reserve, an area of dry sclerophyll forest that connects with other protected areas to the west of Canberra, including Black Mountain Reserve, which is perhaps the best studied location for insects in Australia, since the Australian National Insect Collection (ANIC) is located at the edge of the reserve.
The surrounding bushland is dominated by Scribbly Gum (Eucalyptus rossii R.T.Baker & H.G.Sm.) and Brittle Gum (Eucalyptus mannifera Mudie).
Methods
The Malaise trap was set up on 23 October 2020. The collecting fluid used is initially 95% isopropyl alchohol but 95% denatured ethanol will also be trialled for a subset of the trap’s operation. Access to pure undenatured ethanol is restricted in Australia, so this will be an opportunity to compare the two readily accessible alternatives and any effect they have on DNA preservation and extraction.
Samples are collected from the trap weekly and processed according to the standard operating procedures for the Global Malaise Trap Program. A large DSLR photo (8688 × 5792) is taken as an overview of each sample in an 11 cm2 specimen sorting tray. A small selection of included insects and other specimens is also imaged using a stereomicroscope. Insects will then be dispatched to the Centre for Biodiversity Genomics in Guelph for high-throughput sequencing and to generate digital records in the Barcode of Life Data Systems (BOLD). At that point, the data should also become visible on my BOLD Australia site.
Basic environmental measurements are also being collected (temperature, humidity, pressure, soil moisture, rainfall, windspeed and direction, sunshine hours and solar radiation) and are summarised on weekly pages. Some values are missing at the start of the project.
Temperature, humidity and soil moisture sensors are placed around the trap itself and not exposed to direct sunlight. Pressure data is collected from 10 m away from the trap. Rainfall and wind gauges are positioned in the most open positions available but will nevertheless be affected by surrounding trees and buildings. Solar radiation is measured using a sensor included in the wind gauge. Sunshine hours are purely indicative, representing the time each day that solar radiation is measured to be greater than 120 W/m2. Mean wind direction and mean wind speed are also to be considered purely indicative.
Sensor values are collected every five minutes and maxima, minima, means and calculated values are presented for the 24-hour period ending at 09:00 each day.
Sunrise and sunset times are sourced from Sunrise-Sunset for each date.
Notes, images and measurements are organised in a summary page for each week (see links listed below under Results). This includes links to associated iNaturalist records and Flickr pages. Results are organised using the Catalogue of Life as the base classification, with missing names added as required. Further images and COI barcode sequences will be added once the samples have been processed at the Centre for Biodiversity Genomics in Guelph, Ontario. All specimens and photos can be browsed by taxonomy on the Araba Bioscan Specimens page.

The first batch of samples (ten weeks, including four from the original location and six from the new location) was sent to the Centre for Biodiversity Genomics in Guelph on 5 January 2021.
Results
- Araba Bioscan 23-30 October 2020
- Araba Bioscan 30 October to 6 November 2020
- Araba Bioscan 6-13 November 2020
- Araba Bioscan SLAM comparison 15-19 November 2020
- Araba Bioscan 13-20 November 2020
- Araba Bioscan 20-27 November 2020 (Original location)
- Araba Bioscan 20-27 November 2020 (New location)
- Araba Bioscan 27 November to 4 December 2020
- Araba Bioscan 4-11 December 2020
- Araba Bioscan 11-18 December 2020
- Araba Bioscan 18-25 December 2020
- Araba Bioscan 25 December 2020 to 1 January 2021
- Araba Bioscan 1-8 January 2021
- Araba Bioscan 8-15 January 2021
- Araba Bioscan 15-22 January 2021
- Araba Bioscan 22-29 January 2021
- Araba Bioscan 29 January to 5 February 2021
- Araba Bioscan 5-12 February 2021
- Araba Bioscan 12-19 February 2021
- Araba Bioscan 19-26 February 2021
- Araba Bioscan 26 February to 5 March 2021
- Araba Bioscan 5-12 March 2021
- Araba Bioscan 12-19 March 2021
- Araba Bioscan 19-26 March 2021
- Araba Bioscan 26 March to 2 April 2021
- Araba Bioscan 2-9 April 2021
- Araba Bioscan 9-16 April 2021
- Araba Bioscan 16-23 April 2021
- Araba Bioscan 23-30 April 2021
- Araba Bioscan 30 April to 7 May 2021
- Araba Bioscan 7-14 May 2021
- Araba Bioscan 14-21 May 2021
- Araba Bioscan 21-28 May 2021
- Araba Bioscan 28 May to 4 June 2021
- Araba Bioscan 4-11 June 2021
- Araba Bioscan 11-18 June 2021
- Araba Bioscan 18-25 June 2021
- Araba Bioscan 25 June to 2 July 2021
- Araba Bioscan 2-9 July 2021
- Araba Bioscan 9-16 July 2021
- Araba Bioscan 16-23 July 2021
- Araba Bioscan 23-30 July 2021
- Araba Bioscan 30 July to 6 August 2021
- Araba Bioscan 6-13 August 2021
- Araba Bioscan 13-20 August 2021
- Araba Bioscan 20-27 August 2021
- Araba Bioscan 27 August to 3 September 2021
- Araba Bioscan 3-10 September 2021
- Araba Bioscan 10-17 September 2021
- Araba Bioscan 17-24 September 2021
- Araba Bioscan 24 September to 1 October 2021
- Araba Bioscan 1-8 October 2021
- Araba Bioscan 8-15 October 2021
- Araba Bioscan 15-22 October 2021
- Araba Bioscan 22-29 October 2021
- Araba Bioscan 29 October to 5 November 2021
- Araba Bioscan 5-12 November 2021
- Araba Bioscan 12-19 November 2021
- Araba Bioscan 19-26 November 2021
- Araba Bioscan 26 November to 3 December 2021
- Araba Bioscan 3-10 December 2021
- Araba Bioscan 10-17 December 2021
- Araba Bioscan 17-24 December 2021
- Araba Bioscan 24-31 December 2021
- Araba Bioscan 31 December 2021 to 7 January 2022
- Araba Bioscan 7-14 January 2022
- Araba Bioscan 14-21 January 2022
- Araba Bioscan 21-28 January 2022
- Araba Bioscan 28 January to 4 February 2022
- Araba Bioscan 4-11 February 2022
- Araba Bioscan 11-18 February 2022
- Araba Bioscan 18-25 February 2022
- Araba Bioscan 25 February to 4 March 2022
- Araba Bioscan 4-11 March 2022 (Failed)
- Araba Bioscan 11-18 March 2022
- Araba Bioscan 18-25 March 2022
- Araba Bioscan 25 March to 1 April 2022
- Araba Bioscan 1-8 April 2022
- Araba Bioscan 8-15 April 2022
- Araba Bioscan 15-22 April 2022
- Araba Bioscan 22-29 April 2022
- Araba Bioscan 29 April to 6 May 2022
- Araba Bioscan 6-13 May 2022
- Araba Bioscan 13-20 May 2022
- Araba Bioscan 20-27 May 2022
- Araba Bioscan 27 May to 3 June 2022
- Araba Bioscan 3-10 June 2022
- Araba Bioscan 10-17 June 2022
- Araba Bioscan 17-24 June 2022
- Araba Bioscan 24 June to 1 July 2022
- Araba Bioscan 1-8 July 2022
- Araba Bioscan 8-15 July 2022
- Araba Bioscan 15-22 July 2022
- Araba Bioscan 22-29 July 2022
- Araba Bioscan 29 July to 5 August 2022
- Araba Bioscan 5-12 August 2022
- Araba Bioscan 12-19 August 2022
- Araba Bioscan 19-26 August 2022
- Araba Bioscan 26 August to 2 September 2022
- Araba Bioscan 2-9 September 2022
- Araba Bioscan 9-16 September 2022
- Araba Bioscan 16-23 September 2022
- Araba Bioscan 23-30 September 2022
- Araba Bioscan 30 September to 7 October 2022
- Araba Bioscan 7-14 October 2022
- Araba Bioscan 14-21 October 2022
- Araba Bioscan 21-28 October 2022
- Araba Bioscan SLAM 22-27 January 2023
- Araba Bioscan SLAM 27 January to 3 February 2023
- Lepidoptera Barcoding in Araba Bioscan
- Araba Bioscan SLAM 3-10 February 2023
- Araba Bioscan SLAM 10-17 February 2023
2 replies on “Araba Bioscan Project”
Dear Sir,
I am writing to you after learning about Malaise Trap on your web site. In 2012, under the USDA Biotech Agricultural Research Project entitled “Molecular Taxonomy and DNA Barcoding of agromyzid leaf miner pests of agricultural crops and their control by natural enemies in Bangladesh” I did my M.Phil. degree research in the Department of Zoology, University of Chittagong, Bangladesh. Later, in 2014, under the project named “Global Malaise Programme (Banglaesh region)” I did my PhD research from same institute. For both academic purpose ” Malaise trap ” was used for specimen collection. In continuation of that work, I am still working on the arthropods of Bangladesh. If you have any research opportunities jointly with me, I kindly request you to let me know.
It is worth mentioning that in order to increase the importance and popularity of Malaise traps in the long term ecological work on the biodiversity of the arthropods of any region, I am trying to make Malaise traps at low cost (40-60 USD) and spread them all over the country under my supervision. I am currently working on some malaise trap samples for University of Guelph, Canada. If you need Malaise traps for your research works, feel free to write to me. If you give me a sample, I will sincerely try to make various research aids including Malaise trap for as little Bangladeshi Taka or USD.
May the tune and soul of Christmas carry love, satisfaction, and harmony to your life. May your life be further glorified by your concentration and sincerity in the present-day convenient research.
Happy Christmas in advance!
Sincerely,
Santosh Mazumdar, Ph.D.
Principal
Ananda Multimedia School & College,
Gouripur Bazar, Cumilla, Bangladesh.
I am sincerely sorry for the typing error : “Global Malaise Programme (Banglaesh region)” will be “Global Malaise Programme (Bangladesh region)” instead.